Banixx Data Study & Analysis For Veterinarians

Banixx is clinically proven to be safe and effective

Banixx and Ocular Tissue: Banixx Classified as a Non-Irritant Feb 22, 2020

Banixx was tested in-vitro to assess the acute ocular toxicity after topical application at the Institute for In Vitro Sciences, Inc., Gaithersburg, MD 20878 Laboratory Project 10874. The purpose of the study was to evaluate the potential acute ocular toxicity of Banixx. To this end the Bovine Corneal Opacity and Permeability Assay (BCOP) was used to assess the potential ocular irritancy of the test article to isolated bovine corneas. The methods and procedures used in this assay were consistent with OECD Test Guideline 437: Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage (2017)[1].

Methodology and Results are summarized below

Test Article & Assay Control Preparation - The test article was administered to the test system without dilution (neat). The positive control (100% ethanol) and the negative control (sterile, deionized water) were tested concurrently.

Exposure Times - Three corneas were incubated in the presence of the test article at 32 ± 1 °C for 10 minutes. Three corneas were incubated in the presence of each control at 32 ± 1 °C for 10 minutes.

Evaluation of Test Results - Table 1 summarizes the results of the BCOP assay for the test article and positive control. The following prediction models were used to evaluate the ocular irritancy potential as described by Sina et al. (1995)[2] for non-regulatory purposes or as described in OECD TG 437 (2017).

IIVS Test Article Number Sponsor’s designation Conc. Exposure Time opacity time OD490 Value in vitro score pH
20AA02 Banixx-BJG451 Neat 10 minutes 0.0 -0.003 0.0 1.0
Positive Control Ethanol Neat 10 minutes, 27.7 0.897 41.4 NA

Summary:  The test article produced an In Vitro Score of 0.0. According to the prediction model presented by Sina et al. (1995), the test article would be considered to have mild ocular irritation potential (i.e., In Vitro Score ≤25). According to OECD TG 437, the test article was considered GHS No Category for eye irritation potential (i.e., In Vitro Score ≤3.0). Download the full study here.

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Veterinarian 5-star Reviews
"My husband Dr. Richard Porter and I own a veterinary clinic. We have used Banixx on surgery sites with tremendous results. Not only did the sites clean up, but there was less irritation and they healed with little or no swelling. We had one of our geldings cut his leg; it was a severe injury. With Banixx, it has healed very fast and was a part of his complete recovery."Porter Ridge Veterinary Clinic / Ashland, Nebraska
"Many products used on other animals cannot be used on cats; however, that is not the case with Banixx™. We have had amazing results on wounds and burns on cats.”Dr. Broadhurst, a feline specialist in Pinehurst, NC
"This equine practice has been familiar with and using Banixx for approximately 4 ½ years. The most common types of cases we have used it for are hoof problems that have an infectious component, topical maintenance for wounds healing by second intention, and flushing infected draining puncture wounds. I feel this product's strong points are: 1) It has good anti-bacterial properties; 2) It is friendly to tissue with little tissue reactivity." Mike Black, DVM / Nebraska Equine Veterinary Clinic
"Just try it and let me know what you think…Banixx". I had just called a member of the Society of Wound Management for advice on a particularly difficult case. I was completely unprepared for the results I saw. This wound was at a complete standstill despite the use of many good products. After beginning Banixx, the wound healed in less than a week. This is the most amazing product I have used on wounds. My clients are as excited as I am. My practice is in a remote rural area and I see lots of bad wounds, many of them chronic. I wish I had this product 30 years ago, and I am grateful that I have it now. I am constantly looking for new ways to use it."Dr. Adrienne Ruby / Teesto, Arizona
"Great product! No odor, no pain, easy to apply. I use this product on wounds large and small, to punctures to burns. Healing time is greatly accelerated!" Ann Turner, DVM / Vass, North Carolina

The Science behind Banixx - Dr. Richard Porter DVM

The basic science regarding wound healing gives Banixx the edge over most wound and hoof treatments today. With regard to Banixx ingredients, Banixx has one active ingredient, stabilized hydronium. The inert ingredient is purified water. Banixx is effective on all types of equine wounds, from acute to chronic. It is also potent for various hoof conditions. Banixx’ unique fighting abilities against bacteria and fungi are twofold. The unique pH and high oxygen levels create an acidic environment which reduces toxicity levels of bacteria while increasing antimicrobial activity. The elevated levels of oxygen complement the acidic environment by further energizing antibacterial activity. The end result enhances epithelization and angiogenesis or advanced wound healing. Keeping the tissue site saturated with Banixx will allow tissue oxygenations for a longer period of time as opposed to a few hours. The powerful combination of high acidity and high oxygen level is ideal for faster wound healing and create an environment that is totally inhospitable to bacteria and fungi. Thus the immune system can spend more time healing the injured tissue rather than expending excessive energy fighting infection.

BANIXX HORSE & PET CARE has been shown to send bacteria packing! Test results confirm that Banixx kills what veterinarians call the “BIG THREE”

  • Salmonella choleraesuis
  • Staphylococcus aureus
  • Pseudomonas aeruginosa

— with a 99.9999% kill rate!

In tests of its Anti-Fungal capabilities, Banixx killed 97% of Aspergillus Niger and 99% of Candida Albicans. All tests were conducted by BioVir Labs, an independent, accredited laboratory. View the DATA and RESULTS below.

Antibacterial and Fungal Reduction Activity of Banixx® Horse & Pet Care (BioVir Laboratories, Inc.) April 3 and June 15, 2006

Antibacterial Properties

In separate tests, one to ten million bacterial organisms of Salmonella choleraesuis, Pseudomonas aeruginosa, and Staphylococcus aureus were added to Banixx and results were tested at 0, 1, 3, 5, and 10 minutes later. All tests were run in triplicate. The results are presented below as kill-curves; that is, they illustrate the reduction of organisms over time of exposure. In all tests, the bacterial organisms were "killed", or reduced, to below the detection limit of 1 cfu per mL within ten minutes. The data is presented below.


Anti-Fungal Properties

In separate tests, 500,000 to one million of Candida albicans and Aspergillus niger spores were added to Banixx and results were tested at 1, 2, 5, and 10 minutes later. All tests were run in triplicate. The results are presented below as kill-curves; that is, they illustrate the reduction of organisms over time of exposure. Candida albicans was reduced by about 99% and Aspergillus niger spores were reduced by < 95% within 10 minutes. The data is presented below.


Other Banixx Studies

Two years of testing by a highly-credentialed research veterinarian, produced credible, documented results that allow us to bring Banixx Horse & Pet Care to market. His findings were published in the July 2005 issue of the Journal of Equine Veterinary Science, the official journal of the World Equine Veterinary Association.

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